Comparative genomic hybridization analysis of human oocytes and polar bodies
نویسندگان
چکیده
منابع مشابه
Fluorescence in situ hybridization and spectral imaging analysis of human oocytes and first polar bodies.
We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18, or 21 in failed-fertilized human oocytes. Although abnormalities involving chromosome 16 showed an age-dependent increase, results for the other chromosomes did not show statistically significant differences among the three age groups, <35 years, 35-39 years, and >39 years. The scoring of four chromosomes is...
متن کاملReliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes.
BACKGROUND Preimplantation Genetic Diagnosis (PGD) using FISH to analyze up to nine chromosomes to discard chromosomally abnormal embryos has resulted in an increase of pregnancy rates in certain groups of patients. However, the number of chromosomes that can be analyzed is a clear limitation. We evaluate the reliability of using comparative genomic hybridization (CGH) to detect the whole set o...
متن کاملMitogenomes of Polar Bodies and Corresponding Oocytes
The objective of the present study was to develop an approach that could assess the chromosomal status and the mitochondrial DNA (mtDNA) content of oocytes and their corresponding polar bodies (PBs) with the goal of obtaining a comparative picture of the segregation process both for nuclear and mtDNA. After Whole Genome Amplification (WGA), sequencing of the whole mitochondrial genome was attem...
متن کاملKaryotyping of human oocytes by chromosomal analysis of the second polar bodies.
This paper describes a method for obtaining metaphase chromosomes from human second polar bodies. The second polar body nucleus was injected into the cytoplasm of an enucleated oocyte, which is activated shortly after injection. When the polar body nucleus is transformed into a haploid pronucleus, treatment with okadaic acid was used to induce premature chromosome condensation. A total of 25 an...
متن کاملCorrection: Increasing Live Birth Rate by Preimplantation Genetic Screening of Pooled Polar Bodies Using Array Comparative Genomic Hybridization
[This corrects the article DOI: 10.1371/journal.pone.0128317.].
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2006
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/del157